ohnosequences!

era7 bioinformatics R&D group

EU outbreak Escherichia coli EHEC: some genes involved in adhesion, colonization, pathogenicity and metal resistance

This strain has many genes involved in adhesion, flagellum and fimbria functions, colonization (invasins), and a set of genes related to secretion systems. Some of them are collected in Table 1.

Table 1: Adhesion related, secretion system and pathogenicity and virulence related proteins

Contig ID

tags

Similar to

Protein names

husec41_c1007

Adhesion

C8ULG1

Predicted fimbrial-like adhesin protein

husec41_c101

Adhesion

C8TPC9

Adhesin AIDA-I

husec41_c1040

Adhesion

C8TNN7

Curlin major subunit CsgA

husec41_c110

Adhesion

C8U1Y5

Predicted fimbrillin

husec41_c117

Adhesion

C8TI10

AidA-I adhesin-like protein

husec41_c123

Adhesion

C8TI54

Predicted fimbrial-like adhesin protein

husec41_c1256

Adhesion

B7LH27

Putative adhesin major subunit pilin

husec41_c128

Adhesion

C8U8H5

Predicted fimbrial-like adhesin protein

husec41_c1306

Adhesion

C8TM57

Predicted fimbrial-like adhesin protein

husec41_c1331

Adhesion

C8TUN4

Adhesin YfaL

husec41_c1427

Adhesion

C8U5A7

Predicted fimbrial-like adhesin protein

husec41_c1427

Adhesion

C8U5A6

Predicted fimbrial-like adhesin protein

husec41_c1503

Adhesion

C6UM96

Predicted fimbrial-like adhesin protein

husec41_c151

Adhesion

C8TNL5

Putative AidA-I adhesin-like protein

husec41_c1510

Adhesion

C8TQI1

AidA-I adhesin-like protein

husec41_c1570

Adhesion

C8TGZ3

Putative Iha adhesin

husec41_c1732

Adhesion

C8TZB2

Putative adhesin

husec41_c1801

Adhesion

C6UM96

Predicted fimbrial-like adhesin protein

husec41_c1871

Adhesion

C8TLM3

Putative adhesin

husec41_c2014

Adhesion

D3GX42

Putative adhesin autotransporter

husec41_c2017

Adhesion

C8TTS6

AidA-I adhesin-like protein

husec41_c202

Adhesion

C8TU34

Predicted fimbrial-like adhesin protein

husec41_c279

Adhesion

C8TI51

Predicted fimbrial-like adhesin protein

husec41_c297

Adhesion

C8TGZ3

Putative Iha adhesin

husec41_c311

Adhesion

C6V2W1

Adhesin

husec41_c353

Adhesion

B7L4E2

Putative uncharacterized protein

husec41_c571

Adhesion

C8UG07

Conserved predicted protein

husec41_c678

Adhesion

C8TUN4

Adhesin YfaL

husec41_c749

Adhesion

C8TJB6

Predicted fimbrial-like adhesin protein SfmA

husec41_c822

Adhesion

C8TKM1

Predicted fimbrial-like adhesin protein

husec41_c92

Adhesion

C8TJB9

Predicted fimbrial-like adhesin protein SfmH

husec41_c98

Adhesion

C8TH66

Predicted fimbrial-like adhesin protein

husec41_rep_c2345

Adhesion

C8TNL5

Putative AidA-I adhesin-like protein

husec41_rep_c2430

Adhesion

C6UM96

Predicted fimbrial-like adhesin protein

husec41_rep_c2436

Adhesion

C8TPC9

Adhesin AIDA-I

husec41_c1650

Flagellum

B7LBH5

Flagella control of anti-sigma factor FlgM secretion into the periplasm

husec41_c106

Flagellum

C8TPF0

Flagellar P-ring protein 2 (Basal body P-ring protein 2)

husec41_c106

Flagellum

C8TPE9

Flagellar L-ring protein 2 (Basal body L-ring protein 2)

husec41_c106

Flagellum

C8TPE8

Flagellar component FlgG of cell-distal portion of basal-body rod

husec41_c106

Flagellum

B7LG11

Flagellar component of cell-proximal portion of basal-body rod

husec41_c106

Flagellum

C8TPE6

Flagellar hook protein FlgE

husec41_c106

Flagellum

C8TPE5

Flagellar hook assembly protein FlgD

husec41_c106

Flagellum

C8TPE4

Flagellar component FlgC of cell-proximal portion of basal-body rod

husec41_c106

Flagellum

C8TPE3

Flagellar component FlgB of cell-proximal portion of basal-body rod

husec41_c106

Flagellum

C8TPE2

Assembly protein FlgA for flagellar basal-body periplasmic P ring

husec41_c106

Flagellum

B7LG04

Export chaperone for FlgK and FlgL

husec41_c1100

Flagellum

B7LG17

Flagellar hook-filament junction protein

husec41_c1100

Flagellum

C8TPF2

Flagellar hook-filament junction protein FlgK

husec41_c1100

Flagellum

B5YVV1

Flagellar rod assembly protein/muramidase FlgJ

husec41_c1317

Flagellum

C8TJW2

EAL domain containing protein involved in flagellar function

husec41_c1479

Flagellum

C8TTB9

Conserved predicted protein

husec41_c1600

Flagellum

Q4W8H8

Flagellar transcriptional regulator FlhD 1

husec41_c1656

Flagellum

D3GXI6

Flagellar hook-basal body complex protein FliE 2

husec41_c178

Flagellum

B7L8T6

Flagellin

husec41_c178

Flagellum

C6UYC8

Flagellar filament capping protein

husec41_c180

Flagellum

C8TTC1

Predicted flagellar export pore protein FlhB

husec41_c180

Flagellum

C8TTC0

Predicted flagellar export pore protein FlhA

husec41_c229

Flagellum

B7L524

Putative flagellin structural protein; putative exported protein

husec41_c27

Flagellum

C8TTM1

Flagellar motor switching and energizing component FliM

husec41_c27

Flagellum

C8TTM0

Flagellar biosynthesis protein FliL

husec41_c27

Flagellum

B7L8V5

Flagellar hook-length control protein

husec41_c27

Flagellum

C8TTL8

Flagellar protein FliJ

husec41_c27

Flagellum

C8TTL7

Flagellum-specific ATP synthase FliI

husec41_c27

Flagellum

B7L8V2

Flagellar biosynthesis protein

husec41_c27

Flagellum

C8TTL5

Flagellar motor switching and energizing component protein FliG

husec41_c27

Flagellum

C8TTL4

Flagellar basal-body MS-ring and collar protein FliF

husec41_c518

Flagellum

C8TTK6

Flagellar protein FliS, potentiates polymerization

husec41_c518

Flagellum

D3GXH8

Flagellar protein FliT

husec41_c591

Flagellum

B7LGV4

Flagellar brake protein YcgR (Cyclic di-GMP binding protein YcgR)

husec41_c677

Flagellum

C8THR0

Predicted lateral flagellar system protein

husec41_c723

Flagellum

C8TTM2

Flagellar motor switching and energizing component FliN

husec41_c723

Flagellum

B7L8V9

Flagellar biosynthesis protein

husec41_c723

Flagellum

B5YRX7

Flagellar biosynthetic protein FliP

husec41_c723

Flagellum

C8TTM5

Flagellar biosynthesis protein FliQ

husec41_c723

Flagellum

B7L8W2

Flagellar biosynthetic protein fliR

husec41_c751

Flagellum

C8TUT4

DNA-binding transcriptional repressor LrhA of flagellar, motility and chemotaxis genes

husec41_c807

Flagellum

C8TMI2

Predicted outer membrane lipoprotein

husec41_c1479

Flagelum

C8UC56

Predicted flagellar export pore protein FlhA

husec41_c1940

immune sistem interaction

B7N797

Surface presentation of antigens protein

husec41_c1047

invasin

D3GYK0

Putative invasin

husec41_c215

invasin

C8THZ4

Putative invasin

husec41_c540

invasin

Q8Z7G3

Putative invasin

husec41_c955

invasin

D3GYK0

Putative invasin

husec41_c1172

pathogenesis

B7LBK5

Putative uncharacterized protein

husec41_c2051

pathogenesis

C8TGA6

Conserved predicted protein

husec41_rep_c2406

pathogenesis

B7LF41

Putative uncharacterized protein

husec41_c1167

Secretion system

C8TGA7

Type III secretion system lipoprotein EprK

husec41_c146

Secretion system

B7LG99

Putative secretion pathway M-type protein, membrane anchored

husec41_c146

Secretion system

B7LGA0

Putative secretion pathway protein, L-type protein

husec41_c146

Secretion system

B7LGA1

Putative type II secretion protein (GspK-like)

husec41_c146

Secretion system

B7LG98

Putative uncharacterized protein

husec41_c146

Secretion system

B7LGA2

Putative type II secretion protein (GspI-like)

husec41_c146

Secretion system

B7LGA4

Putative uncharacterized protein

husec41_c146

Secretion system

B7LGA5

Putative general secretion pathway protein G (EpsG-like)

husec41_c146

Secretion system

B7LGA3

General secretion pathway protein F

husec41_c146

Secretion system

D3GVL9

Type II secretion system protein E

husec41_c146

Secretion system

B7LGA8

General secretion pathway protein D

husec41_c146

Secretion system

B7LGA9

Putative secretion pathway protein, C-type protein

husec41_c1468

Secretion system

D8AT29

Type III secretion system protein PrgH-EprH

husec41_c1538

Secretion system

B7LF39

Putative Type III secretion EprH protein

husec41_c1598

Secretion system

C8TGB3

Type III secretion protein EpaQ

husec41_c1598

Secretion system

B7LF43

Putative Type III secretion protein EpaR

husec41_c16

Secretion system

D3GUQ5

Microcin H47

husec41_c16

Secretion system

D3GUQ6

Microcin H47 immunity protein

husec41_c1639

Secretion system

C8TGF0

T3SS effector-like protein EspX-homolog

husec41_c1643

Secretion system

E9Z029

DotU family protein type IV/VI secretion system protein

husec41_c1674

Secretion system

C8U8E4

T3SS effector-like protein EspR-homolog

husec41_c1783

Secretion system

D3GUZ5

Putative type VI secretion protein

husec41_c1791

Secretion System

Q3SBC5

EpaS1

husec41_c1791

Secretion system

B7LF43

Putative Type III secretion protein EpaR

husec41_c1849

Secretion system

D3GV03

Putative type VI secretion protein

husec41_c1983

Secretion system

D3GV03

Putative type VI secretion protein

husec41_c2008

Secretion system

D3GV03

Putative type VI secretion protein

husec41_c2036

Secretion system

D3GV03

Putative type VI secretion protein

husec41_c2178

Secretion system

D3GUY9

Putative type VI secretion protein

husec41_c323

Secretion system

D3GUZ0

Putative type VI secretion protein

husec41_c323

Secretion system

D3GUZ1

Putative type VI secretion protein

husec41_c333

Secretion system

C8THD4

Assembly protein HofC in type IV pilin biogenesis, transmembrane protein

husec41_c343

Secretion system

C8TKE9

Sec-independent protein translocase protein tatA/E homolog 1

husec41_c539

Secretion System

Q3SBB5

EpaO

husec41_c542

Secretion System

B7LF47

Putative type III secretion system protein EpaP

husec41_c576

Secretion system

B5YZD3

Secretion monitor protein

husec41_c716

Secretion system

C8TIZ8

SecYEG protein translocase auxillary subunit SecD

husec41_c716

Secretion system

C8TIZ9

SecYEG protein translocase auxillary subunit SecF

husec41_c72

Secretion system

D3GUQ1

Probable microcin H47 secretion/processing ATP-binding protein (EC 3.4.22.-)

husec41_c734

Secretion system

C8TIZ8

SecYEG protein translocase auxillary subunit SecD

husec41_c734

Secretion system

C8TIZ7

SecYEG protein translocase auxillary subunit

husec41_c771

Secretion system

D3GU38

Putative type VI secretion protein

husec41_c813

Secretion system

C8UKR4

T3SS effector-like protein EspL-homolog

husec41_c854

Secretion system

D3GSV7

Type III secretion system protein

husec41_c854

Secretion system

C8UAK5

Type III secretion protein EprJ

husec41_c862

Secretion system

D3GUY9

Putative type VI secretion protein

husec41_c929

Secretion system

D3GUZ5

Putative type VI secretion protein

husec41_c975

Secretion system

C8TKY1

Sec-independent protein translocase protein tatA/E homolog 2

husec41_c335

Secretion system?

C8TVQ3

Predicted transglycosylase

husec41_c553

Secretion System?

B7L5T3

Putative HlyD family secretion protein

husec41_c902

virulence, plasmid

D3H5A9

Virulence protein required for expression/correct membrane localisation of IcsA (VirG)

husec41_c1048

fimbria

B7L7K6

Putative major fimbrial subunit FmlA

husec41_c1130

fimbria

B7LAC4

Putative uncharacterized protein ybgQ

husec41_c1130

fimbria

C8TKM4

Putative fimbrial-like protein

husec41_c123

fimbria

C8TI53

Predicted outer membrane protein

husec41_c1256

fimbria

C8THB2

Putative fimbrial protein

husec41_c1279

fimbria

C8U6Q7

Putative fimbrial subunit protein

husec41_c128

fimbria

B7L7K6

Putative major fimbrial subunit FmlA

husec41_c128

fimbria

C8TQ64

Outer membrane usher protein FimD

husec41_c128

fimbria

B7L7K2

Putative fimbrial-like adhesin exported protein

husec41_c128

fimbria

B7L7K1

Putative fimbrial-like exported adhesin protein

husec41_c1369

fimbria

C8TL83

Putative fimbrial protein

husec41_c1400

fimbria

C8U6Q8

Putative fimbrial subunit protein

husec41_c1400

fimbria

B7LBJ0

Putative minor fimbrial subunit

husec41_c1503

fimbria

C8THG4

Putative fimbrial protein

husec41_c1503

fimbria

C8THG3

Putative fimbrial protein

husec41_c1527

fimbria, plasmid

P46005

Outer membrane usher protein AggC

husec41_c1592

fimbria

C8TMJ0

Putative fimbrial usher protein

husec41_c1651

fimbria

C8TUY3

Putative fimbrial-like protein

husec41_c1651

fimbria

C8TUY2

Outer membrane usher protein

husec41_c1675

fimbria

D3H3A6

Fimbrial outer membrane usher protein

husec41_c1701

fimbria, plasmid

P46005

Outer membrane usher protein AggC

husec41_c1953

fimbria, plasmid

B7LWW0

14 kDa aggregative adherence fimbriae I protein (Modular protein)

husec41_c202

fimbria

C8TU35

Putative outer membrane protein

husec41_c202

fimbria

B7L9Y3

Putative fimbrial-like adhesin protein

husec41_c2205

fimbria, plasmid

P46005

Outer membrane usher protein AggC

husec41_c279

fimbria

C8TI53

Predicted outer membrane protein

husec41_c353

fimbria

D3H3A6

Fimbrial outer membrane usher protein

husec41_c487

fimbria

B7L8D9

Putative fimbrial-like adhesin protein

husec41_c498

fimbria

C8TUY2

Outer membrane usher protein

husec41_c507

fimbria, plasmid

P46005

Outer membrane usher protein AggC

husec41_c514

fimbria

C8THG6

Probable outer membrane porin protein

husec41_c636

fimbria

C8TMJ2

Putative fimbrial adhesin protein

husec41_c636

fimbria

C8TMJ1

Putative minor fimbrial subunit

husec41_c636

fimbria

C8TM56

Putative outer membrane usher protein

husec41_c636

fimbria

C8TMI8

Putative fimbrial major protein

husec41_c679

fimbria

C8THG8

Putative fimbrial-like protein

husec41_c775

fimbria, plasmid

P46005

Outer membrane usher protein AggC

husec41_c777

fimbria

C8THG4

Putative fimbrial protein

husec41_c777

fimbria

C8U1K8

Putative fimbrial protein

husec41_c777

fimbria

C8THG6

Probable outer membrane porin protein

husec41_c92

fimbria

C8TQ64

Outer membrane usher protein FimD

husec41_c972

Fimbria

Q8Z2Q1

Probable fimbrial subunit protein

husec41_c98

fimbria

C8TH67

Predicted outer membrane usher protein

husec41_rep_c2402

fimbria

D3GT73

Putative fimbrial protein



Genes involved in metal resistance

This strain has a set of genes involved in metal resistance.

It could bear a mercuric resistance plasmid. The predicted proteins included in Table 2 are all located in the same contig and probably are in a plasmid forming a functional operon with a MerR family regulator in a divergent orientation to the rest of the components of the operon.

Table 2:  Mercury resistance plasmid genes

Contig ID

tags

Similar to

Protein names

husec41_c784

Mercuric resistance

C8UQM8

Mercuric resistance operon regulatory protein MerR

husec41_c784

Mercuric resistance, plasmid

C8UQM9

Mercuric ion transport protein MerT

husec41_c784

Mercuric resistance

D3H375

Mercuric ion transport protein

husec41_c784

Mercuric resistance, plasmid

Q0ZKU6

Mercuric resistance protein MerC (Mercury resistance operon transport protein MerC) (Putative uncharacterized protein)

husec41_c784

Mercuric

Q935L3

Putative mercuric reductase (EC 1.16.1.1)

husec41_c784

Mercuric resistance

D3H372

MerR-family transcriptional regulator

husec41_c784

plasmid

Q5J458

Urf2

Table 3 collects the genes involved in Tellurium resistance.


Table 3: Tellurium resistance genes

Contig ID

tags

Similar to

Protein names

husec41_c1152

Tellurium

C8TNH6

Putative tellurium resistance protein TerC

husec41_c1243

Tellurium

C8TNH3

Putative tellurium resistance protein TerZ

husec41_c1243

Tellurium

C8TNH4

Putative tellurium resistance protein TerA

husec41_c1243

Tellurium

C8TNH5

Putative tellurium resistance protein TerB

husec41_c1512

Tellurium

C8TX21

Putative tellurium resistance protein TerF

husec41_c1713

Tellurium

C8TNH6

Putative tellurium resistance protein TerC

husec41_c1713

Tellurium

C8TNH7

Putative tellurium resistance protein TerD

husec41_c273

Tellurium resistance

Q19NM8

TerY3

husec41_c2960

Tellurium resistance, plasmid

Q19NL2

TerE

husec41_c750

Tellurium resistance

Q19NM7

TerY2

husec41_c750

Tellurium resistance, plasmid

P75012

Tellurium resistance protein TerX

husec41_c750

Tellurium resistance

Q19NM5

TerY1

husec41_c750

Tellurium resistance, plasmid

P75010

Tellurium resistance protein terW

husec41_c794

Tellurium

C8TNH8

Putative tellurium resistance protein TerE

In addition to genes involved in Mercury resistance and Tellurium resistance we have predicted and annotated in this genome many genes involved in resistance to other metals as Cobalt and Copper. See complete annotation at: