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era7 bioinformatics R&D group

EU outbreak Escherichia coli EHEC genome plasticity

transposases

It seems that there are 121 putative transposases in this genome. It probably implies a high genomic plasticity and flexibility for adaptation to changing environments.

Table 1: Transposases

Contig ID

tags

Similar to

Protein names

husec41_c1004

Transposase

D3H358

Transposase

husec41_c1066

Transposase

P30192

Putative uncharacterized protein ychG

husec41_c1147

Transposase

B7L8U5

Putative transposase ORF A, IS609 family

husec41_c1214

Transposase

C2DME2

Possible transposase insP for IS630

husec41_c130

Transposase

B7L5X1

Tn7-like transposition protein TnsB

husec41_c130

Transposase

B7L5W7

Tn7-like transposition protein TnsC

husec41_c1390

Transposase

E9Z073

Transposase

husec41_c1412

Transposase

C8TNV4

Predicted transposase

husec41_c1539

Transposase

Q8XC14

Putative IS encoded protein encoded within prophage CP-933O

husec41_c1554

Transposase

P16943

Insertion element IS630 uncharacterized 39 kDa protein (ISO-IS200 39 kDa protein)

husec41_c1562

Transposase, plasmid

B7LWU0

Transposon Tn3 resolvase

husec41_c1562

Transposase, plasmid

C7S9E2

Transposase for transposon Tn3 (Truncated TnpA)

husec41_c1569

Transposase

B3HPU8

Transposase OrfA, ISEc8

husec41_c1595

Transposase

Q6Q6S9

Transposase

husec41_c1628

Transposase

B7LBW1

Transposase

husec41_c1683

Transposase

B7LHE6

Putative uncharacterized protein yncI

husec41_c1687

Transposase

B3HAL5

Transposon Tn21 resolvase

husec41_c1689

Transposase

P03008

Transposase for transposon Tn3

husec41_c1781

Transposase

B7LFE0

IS3 element protein InsE

husec41_c1781

Transposase, plasmid

B7LA99

IS3 element protein InsF (Transposase ORF B, IS3)

husec41_c1799

Transposase

C8TKW2

Putative uncharacterized protein

husec41_c1859

Transposase

B6IAL2

H repeat-associated protein

husec41_c1864

Transposase, plasmid

C9WXH4

Transposase (Fragment)

husec41_c1864

Transposase, plasmid

Q935I2

Putative transposase

husec41_c188

Transposase

D3GR79

Putative transposase

husec41_c188

Transposase

D3GR79

Putative transposase

husec41_c1905

Transposase, plasmid

B7LWW4

Transposase ORF A, IS1

husec41_c1921

Transposase

D3H358

Transposase

husec41_c1974

Transposase

B7LWV0

Transposase ORF A, IS629

husec41_c1974

Transposase, plasmid

B7LWV1

Putative uncharacterized protein

husec41_c199

Transposase

P03008

Transposase for transposon Tn3

husec41_c1995

Transposase

B7LFN9

Transposase ORF A, IS3 family

husec41_c1998

Transposase

D3GV20

Transposase (Fragment)

husec41_c2024

Transposase

B5AXD2

Putative transposase

husec41_c2049

Transposase

Q6Q6S9

Transposase

husec41_c2055

Transposase

A8A1D5

Transposase, IS605 family

husec41_c2063

Transposase

B7L8E6

Putative transposase

husec41_c2064

Transposase

E7H9R9

Transposase IS66 family protein

husec41_c2066

Transposase

B7L5X1

Tn7-like transposition protein TnsB

husec41_c211

Transposase

B7L4U1

Putative transposase

husec41_c2125

Transposase, plasmid

B7LA98

Putative uncharacterized protein

husec41_c2125

Transposase

D3GV17

Transposase

husec41_c2125

Transposase, plasmid

B7LX19

Putative transposase, IS110 family

husec41_c2132

Transposase

B7LFP9

Transposase ORF B, IS629

husec41_c2176

Transposase

E9Z008

Transposase

husec41_c2181

Transposase

B7LFP9

Transposase ORF B, IS629

husec41_c2203

Transposase, plasmid

F2W481

ISL3 family transposase

husec41_c226

Transposase, plasmid

B7UHC5

Transposase of ISEc13 of IS110 family (Transposase of ISEc21)

husec41_c226

Transposase

B3IA39

Truncated transposase

husec41_c230

Transposase

B7LBF9

Putative transposase

husec41_c273

Transposase

C4HU49

Transposase

husec41_c30

Transposase

P03008

Transposase for transposon Tn3

husec41_c30

Transposase, plasmid

D9Z5B6

TnpA

husec41_c37

Transposase

D7ZCL8

Transposase, IS4 family (Fragment)

husec41_c42

Transposase

A7ZMR8

Transposase, IS605 family

husec41_c524

Transposase, plasmid

C7S9T2

Transposon Tn21 modulator protein

husec41_c524

Transposase

D3H359

Transposon Tn21 resolvase

husec41_c524

Transposase

D3H358

Transposase

husec41_c526

Transposase

D3GX03

Insertion sequence IS100, ATP-binding protein

husec41_c526

Transposase

P59697

Transposase for insertion sequence element IS200

husec41_c6

Transposase, plasmid

D3H553

Transposase

husec41_c642

Transposase

B3WUE9

TniA transposase protein

husec41_c66

Transposase, plasmid

Q0H058

IS1N transposase

husec41_c662

Transposase

Q19NI3

TnsD

husec41_c699

Transposase?

B7LHE6

Putative uncharacterized protein yncI

husec41_c826

Transposase

D8AYZ0

Transposase

husec41_c826

Transposase

B7L939

Putative transposase ORF 2, IS66 family

husec41_c835

Transposase

B7LDR0

Transposase ORF B, IS1

husec41_c838

Transposase

E7SID2

Transposase

husec41_c87

Transposase

B7L5X0

Tn7-like transposase TnsA

husec41_c885

Transposase

E9Z073

Transposase

husec41_c887

Transposase

C8TWQ4

Predicted IS602 transposase OrfB

husec41_rep_c2269

Transposase

C8TGN1

Putative IS621 transposase

husec41_rep_c2271

Transposase

P11901

Transposase for insertion sequence element IS421

husec41_rep_c2273

Transposase

E1U309

Transposase InsAB’

husec41_rep_c2275

Transposase, plasmid

Q7AQT7

Putative transposase

husec41_rep_c2276

Transposase

B7LDT8

Putative uncharacterized protein

husec41_rep_c2286

Transposase

B7L8E6

Putative transposase

husec41_rep_c2306

Transposase

E9YLK7

Transposase (Fragment)

husec41_rep_c2315

Transposase

E9YLK7

Transposase (Fragment)

husec41_rep_c2317

Transposase

Q8XC14

Putative IS encoded protein encoded within prophage CP-933O

husec41_rep_c2317

Transposase

C6UPH5

Transposase ISEc8 (Transposase, ISEc8)

husec41_rep_c2321

Transposase

C8UCH9

Putative uncharacterized protein

husec41_rep_c2323

Transposase

C8TND9

Putative uncharacterized protein

husec41_rep_c2336

Transposase

A8A1D5

Transposase, IS605 family

husec41_rep_c2336

Transposase

C8TP26

Putative IS609 transposase TnpB

husec41_rep_c2356

Transposase

B7L8Y0

IS30 transposase; KpLE2 phage-like element

husec41_rep_c2370

Transposase

Q8VRB2

Putative transposase

husec41_rep_c2372

Transposase

B7L5W7

Tn7-like transposition protein TnsC

husec41_rep_c2408

Transposase

E8IR83

IS66 transposase

husec41_rep_c2429

Transposase

P03008

Transposase for transposon Tn3

husec41_rep_c2437

Transposase, plasmid

B7LWW4

Transposase ORF A, IS1

husec41_rep_c2441

Transposase

D3GV27

Transposase

husec41_rep_c2445

Transposase

B7LDR0

Transposase ORF B, IS1

husec41_rep_c2446

Transposase, plasmid

B7LWV1

Putative uncharacterized protein

husec41_rep_c2449

Transposase

D3GV14

Transposase

husec41_rep_c2478

Transposase

E7IJ32

Putative transposase

husec41_rep_c2487

Transposase

E1U309

Transposase InsAB’

husec41_rep_c2498

Transposase

B7LFP9

Transposase ORF B, IS629

husec41_rep_c2499

Transposase

E3Y631

ISL3 family transposase domain protein

husec41_rep_c2515

Transposase

Q2EEQ8

Putative defective transposase ybfQ

husec41_rep_c2516

Transposase

E3XVP6

Putative transposase

husec41_rep_c2520

Transposase

B6ICP6

Truncated transposase

husec41_rep_c2540

Transposase

P76102

Putative transposase InsQ for insertion sequence element IS609

husec41_rep_c2544

Transposase

D6HU78

Putative uncharacterized protein

husec41_rep_c2565

Transposase

B7L6S2

Putative transposase

husec41_rep_c2570

Transposase

D6HU78

Putative uncharacterized protein

husec41_rep_c2572

Transposase

C8ULY2

Putative IS609 transposase TnpA

husec41_rep_c2577

Transposase

Q4E6D2

Transposase (IS4 family)

husec41_rep_c2578

Transposase

E3Y0F9

Transposase, IS605 OrfB family

husec41_rep_c2639

Transposase

B7LBV5

Transposase, IS110 family

husec41_rep_c2668

Transposase

E9YA15

Transposase

husec41_rep_c2679

Transposase

E8Y4A6

Transposase IS200-family protein

husec41_rep_c2743

Transposase, plasmid

B5YPI1

ISSd1, transposase OrfB

husec41_rep_c2812

Transposase

B7L6S2

Putative transposase

husec41_rep_c2873

Transposase

Q8VRB2

Putative transposase

husec41_rep_c2875

Transposase

P0CE57

Transposase insH for insertion sequence element IS5R

husec41_rep_c2889

Transposase

D8B2Q9

ISPsy10, transposase family protein (Fragment)

husec41_rep_c2890

Transposase

B1LJZ9

IS5 transposase

husec41_rep_c2903

Transposase

D6HU78

Putative uncharacterized protein

husec41_rep_c2907

Transposase

B7L6S2

Putative transposase

In the next table we have collected some comments about these set of transposases of E. coli EHEC genome.

UNIPROT

Annotation

COMMENTS

FAMILY

D3H358

Transposase

Transposase_7. Segun interpro

Tn3

P30192

Putative uncharacterized protein ychG

Caution,  it can be a pseudogene

B7L8U5

Putative transposase ORF A, IS609 family

Putative

IS609

C2DME2

Possible transposase insP for IS630

Length 115 aa

IS630

E9Z073

Transposase

REP-associated transposases (Pubmed: 16563168)

IS110

C8TNV4

Predicted transposase

ISL3

B7LWU0

Transposon Tn3 resolvase

On plasmid, in some cases is associated to mercury resistance

Tn3

C7S9E2

Transposase for transposon Tn3 (Truncated TnpA)

125 aa on plasmid ?

Tn3

B3HPU8

Transposase OrfA, ISEc8

120 aa

ISEc8

Q6Q6S9

Transposase

Molecular characterization of cefoxitin-resistant Escherichia coli from Canadian hospitals

IS911

B7LBW1

Transposase

Pubmed 19165319

B7LHE6

Putative uncharacterized protein yncI

REP-associated transposases (Pubmed: 16563168)

IS4

P03008

Transposase for transposon Tn3

Tn3

B7LFE0

IS3 element protein InsE

Pubmed  19165319

IS3

B7LA99

IS3 element protein InsF (Transposase ORF B, IS3)

IS3

C8TKW2

Putative uncharacterized protein

IS66

B6IAL2

H repeat-associated protein

REP-associated transposases (Pubmed: 16563168)

IS4

C9WXH4

Transposase (Fragment)

Tni?

Q935I2

Putative transposase

IS26

D3GR79

Putative transposase

D3GR79

Putative transposase

B7LWW4

Transposase ORF A, IS1

REP-associated transposases (Pubmed: 16563168)

IS110

D3H358

Transposase

On plasmid, in some cases is associated to mercury resistance

Tn3

B7LWV0

Transposase ORF A, IS629

IS629

B7LWV1

Putative uncharacterized protein

P03008

Transposase for transposon Tn3

On plasmid, in some cases is associated to mercury resistance

Tn3

B7LFN9

Transposase ORF A, IS3 family

IS911

D3GV20

Transposase (Fragment)

Mutator

B5AXD2

Putative transposase

IS30

Q6Q6S9

Transposase

IS911

A8A1D5

Transposase, IS605 family

IS609

B7L8E6

Putative transposase

ISEc3

E7H9R9

Transposase IS66 family protein

IS66

B7L5X1

Tn7-like transposition protein TnsB

Tn7

B7L4U1

Putative transposase

YhgA

B7LA98

Putative uncharacterized protein

D3GV17

Transposase

IS66

B7LX19

Putative transposase, IS110 family

IS116/IS110/IS902 family REP-associated transposases (Pubmed: 16563168)

IS110

B7LFP9

Transposase ORF B, IS629

IS629

E9Z008

Transposase

B7LFP9

Transposase ORF B, IS629

IS629

F2W481

ISL3 family transposase

ISL3

B7UHC5

Transposase of ISEc13 of IS110 family (Transposase of ISEc21)

IS116/IS110/IS902  family REP-associated transposases (Pubmed: 16563168)

IS110

B3IA39

Truncated transposase

B7LBF9

Putative transposase

YhgA

C4HU49

Transposase

P03008

Transposase for transposon Tn3

On plasmid, in some cases is associated to mercury resistance

Tn3

D9Z5B6

TnpA

ISEcp1  TnpA

D7ZCL8

Transposase, IS4 family (Fragment)

REP-associated transposases (Pubmed: 16563168)

IS4

A7ZMR8

Transposase, IS605 family

IS605

D3H358

Transposase

In some cases is associated to mercury resistance

Tn3

P59697

Transposase for insertion sequence element IS200

TpnA2 related

IS200

D3H553

Transposase

IS66

B3WUE9

TniA transposase protein

Q0H058

IS1N transposase

ISN1

B7LHE6

Putative uncharacterized protein yncI

REP-associated transposases (Pubmed: 16563168)

IS4

D8AYZ0

Transposase

B7L939

Putative transposase ORF 2, IS66 family

IS66

B7LDR0

Transposase ORF B, IS1

IS1

E7SID2

Transposase

B7L5X0

Tn7-like transposase TnsA

Tn7-like

E9Z073

Transposase

REP-associated transposases (Pubmed: 16563168)

IS110

C8TWQ4

Predicted IS602 transposase OrfB

IS911

C8TGN1

Putative IS621 transposase

IS116/IS110/IS902 family

IS621

P11901

Transposase for insertion sequence element IS421

IS4 related

IS186

E1U309

Transposase InsAB’

IS1

Q7AQT7

Putative transposase

IS1216 related

TnpA  - IS26

B7LDT8

Putative uncharacterized protein

ISSd1 related

IS600

B7L8E6

Putative transposase

ISEc3

E9YLK7

Transposase (Fragment)

E9YLK7

Transposase (Fragment)

C6UPH5

Transposase ISEc8 (Transposase, ISEc8)

IS66

C8UCH9

Putative uncharacterized protein

IS682 related

IS66

C8TND9

Putative uncharacterized protein

ISEc8 ??

A8A1D5

Transposase, IS605 family

IS609

C8TP26

Putative IS609 transposase TnpB

IS605

B7L8Y0

IS30 transposase; KpLE2 phage-like element

IS30

Q8VRB2

Putative transposase

E8IR83

IS66 transposase

IS66

P03008

Transposase for transposon Tn3

Tn3 - TnpA

B7LWW4

Transposase ORF A, IS1

IS1

D3GV27

Transposase

IS30

B7LDR0

Transposase ORF B, IS1

IS1

B7LWV1

Putative uncharacterized protein

D3GV14

Transposase

IS116/IS110/IS902 family

IS110

E7IJ32

Putative transposase

E1U309

Transposase InsAB’

IS1

B7LFP9

Transposase ORF B, IS629

IS629

E3Y631

ISL3 family transposase domain protein

ISL3

Q2EEQ8

Putative defective transposase ybfQ

ISEc2

E3XVP6

Putative transposase

B6ICP6

Truncated transposase

P76102

Putative transposase InsQ for insertion sequence element IS609

IS605

D6HU78

Putative uncharacterized protein

B7L6S2

Putative transposase

IS605

D6HU78

Putative uncharacterized protein

C8ULY2

Putative IS609 transposase TnpA

IS 200 related

IS609

Q4E6D2

Transposase (IS4 family)

InsH  -   IS4  related REP-associated transposases (Pubmed: 16563168)

IS5

E3Y0F9

Transposase, IS605 OrfB family

IS605

B7LBV5

Transposase, IS110 family

IS116/IS110/IS902

IS110

E9YA15

Transposase

IS1 1/5/6

E8Y4A6

Transposase IS200-family protein

IS200

B5YPI1

ISSd1, transposase OrfB

ISSd1

B7L6S2

Putative transposase

IS 609 related

IS605

Q8VRB2

Putative transposase

P0CE57

Transposase insH for insertion sequence element IS5R

REP-associated transposases (Pubmed: 16563168)

IS5

D8B2Q9

ISPsy10, transposase family protein (Fragment)

ISpsy10

B1LJZ9

IS5 transposase

REP-associated transposases (Pubmed: 16563168)

IS5

D6HU78

Putative uncharacterized protein

B7L6S2

Putative transposase

IS 609 related

IS605

Plasmids

Around 246 predicted proteins appear to be related with plasmids. See the genome annotation table with functional tags at:

http://www.era7bioinformatics.com/docs/EHEC_E_COLI_GERMANY_OUTBREAK_Annotation_Era7Bioinformatics_v1562011.xls

This strain has many genetic capabilities that probably confer it a competitive advantage and a high capability for environment adaptation. Some important features that this strain bears in its genome:

  • A restriction-modification system
  • ­Many proteins involved in Fe transport and utilization. Siderophores: aerobactin, enterobactin.
  • Lysozyme
  • ­A general inhibitor of pancreatic serine proteases: inhibits chymotrypsin, trypsin, elastases, factor X, kallikrein as well as a variety of other proteases
  • Proteins involved in anaerobic respiration
  • ­Antimicrobial peptides
  • ­Proteins involved in quorum-sensing and biofilm formation
  • ­Proteins involved in Ni, Cu, Zn and Co resistance
  • ­More tham 170 phage proteins